Danish
Uniprot ID:  O64989
  • Protein: Steroid (22S)-hydroxylase
  • Organism: Mouse-ear cress (Arabidopsis thaliana)
  • Length: 513
  • Sequence:
    • MFETEHHTLLPLLLLPSLLSLLLFLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDKPFAFPFVDFPNGLPIRVSRIL
Exclude chains with
Mutations (vs. UniProt)
Clear
Number of chains:  4
PDB ID:  pdb_00006a15 , Chain A 
Resolution 1.79 Å
Residue indices (Uniprot), coverage 35-516 (29-513), 0.88
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine
Mutations (UniProt numbering) L28A, P506L
Insertions 513insHHHH
Deletions F2-L27, E256-V277, N434-S446
Sequence identity to UniProt 0.9956
auth_asym_id A
Missing residues 46TRP-48PHE:280GLN-281ARG:434THR-436GLY
Structural gaps Exists
label_asym_ids of heme B
Available structure PDB and Modeled
RMSD of experimental residues after energy minimization 0.11 Å
PDB ID:  pdb_00006a16 , Chain A 
Resolution 2.00 Å
Residue indices (Uniprot), coverage 36-513 (29-513), 0.95
Difference from Uniprot sequence engineered mutation:expression tag:initiating methionine
Mutations (UniProt numbering) L28A, P506L
Insertions 513insHHHH
Deletions F2-L27
Sequence identity to UniProt 0.9959
auth_asym_id A
Structural gaps Exists
label_asym_ids of heme B
Available structure PDB
PDB ID:  pdb_00006a17 , Chain A 
Resolution 2.30 Å
Residue indices (Uniprot), coverage 36-513 (29-513), 0.88
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine
Mutations (UniProt numbering) L28A, P506L
Insertions 513insHHHH
Deletions F2-L27, E256-V277, N435-S446
Sequence identity to UniProt 0.9956
auth_asym_id A
label_asym_ids of heme C
Available structure PDB
PDB ID:  pdb_00006a18 , Chain A 
Resolution 2.48 Å
Residue indices (Uniprot), coverage 37-513 (29-513), 0.88
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine
Mutations (UniProt numbering) L28A, P506L
Insertions 513insHHHH
Deletions F2-L27, E256-V277, N435-S446
Sequence identity to UniProt 0.9956
auth_asym_id A
Missing residues 219ALA-222ASN
Structural gaps Exists
label_asym_ids of heme B
Available structure PDB and Modeled
RMSD of experimental residues after energy minimization 0.10 Å