Danish
Uniprot ID:  P00388
  • Protein: NADPH--cytochrome P450 reductase
  • Organism: Rat (Rattus norvegicus)
  • Length: 678
  • Sequence:
    • MGDSHEDTSATMPEAVAEEVSLFSTTDMVLFSLIVGVLTYWFIFRKKKEEIPEFSKIQTTAPPVKESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDKSLVVFCMATYGEGDPTDNAQDFYDWLQETDVDLTGVKFAVFGLGNKTYEHFNAMGKYVDQRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEFFGVEATGEESSIRQYELVVHEDMDVAKVYTGEMGRLKSYENQKPPFDAKNPFLAAVTANRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQIGEILGADLDVIMSLNNLDEESNKKHPFPCPTTYRTALTYYLDITNPPRTNVLYELAQYASEPSEQEHLHKMASSSGEGKELYLSWVVEARRHILAILQDYPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVAVEYEAKSGRVNKGVATSWLRAKEPAGENGGRALVPMFVRKSQFRLPFKSTTPVIMVGPGTGIAPFMGFIQERAWLREQGKEVGETLLYYGCRRSDEDYLYREELARFHKDGALTQLNVAFSREQAHKVYVQHLLKRDREHLWKLIHEGGAHIYVCGDARNMAKDVQNTFYDIVAEFGPMEHTQAVDYVKKLMTKGRYSLDVWS
Exclude chains with
Mutations (vs. UniProt)
Clear
Number of chains:  8
PDB ID:  pdb_00006j7a , Chain A 
Resolution 3.27 Å
Residue indices (Uniprot), coverage 10-855 (58-678), 0.99
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine:linker
Mutations (UniProt numbering) D3S, D7A, S9K, A10E, T11V, M12H, P13I, E14R, V16E, A17N, E18S, V20F, S21M, L22R, F23N, S24F, T25Q, T26K, D27G, M28Q, L30S, F31R, S32E, L33G, I34F, V35K, G36L, L38M, T39A, Y40S, W41L, F42Y, I43H, F44I, R45Y, K46T, K47A, K48L, I51Q, P52T, S55L, K56R, I57M
Insertions 5insHHHHHSSGLVPRGSHMERPQLDSMSQDLSEALK, 50insEIERNKQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEAIPYTPATQHYVKRLHEVGGTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPSIDNPTKFKQLYRARMNTLEMTPEVKHRVTEEAKTAFLLNIELFEELQALLPEEHKDQSAS
Deletions T236-E239
Sequence identity to UniProt 0.9362
auth_asym_id A
Structural gaps Exists
label_asym_ids of heme C
Available structure PDB
PDB ID:  pdb_00006j7a , Chain B 
Resolution 3.27 Å
Residue indices (Uniprot), coverage 10-855 (58-678), 0.99
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine:linker
Mutations (UniProt numbering) D3S, D7A, S9K, A10E, T11V, M12H, P13I, E14R, V16E, A17N, E18S, V20F, S21M, L22R, F23N, S24F, T25Q, T26K, D27G, M28Q, L30S, F31R, S32E, L33G, I34F, V35K, G36L, L38M, T39A, Y40S, W41L, F42Y, I43H, F44I, R45Y, K46T, K47A, K48L, I51Q, P52T, S55L, K56R, I57M
Insertions 5insHHHHHSSGLVPRGSHMERPQLDSMSQDLSEALK, 50insEIERNKQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEAIPYTPATQHYVKRLHEVGGTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPSIDNPTKFKQLYRARMNTLEMTPEVKHRVTEEAKTAFLLNIELFEELQALLPEEHKDQSAS
Deletions T236-E239
Sequence identity to UniProt 0.9362
auth_asym_id B
Structural gaps Exists
label_asym_ids of heme F
Available structure PDB
PDB ID:  pdb_00006j7i , Chain A 
Resolution 3.30 Å
Residue indices (Uniprot), coverage 10-853 (58-678), 0.99
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine:linker
Mutations (UniProt numbering) D3S, E6R, D7M, T8N, S9T, A10L, T11E, P13T, E14P, A15E, A17K, E18H, E19R, S21T, L22E, F23E, S24A, T25K, D27A, M28F, V29L, F31N, S32I, L33E, I34L, V35F, G36E, V37E, T39Q, Y40A, W41L, F42L, I43P, F44E, R45E, K46H, K48D, E49Q, E50S, I51A, P52S, E53Q, F54T, S55E, K56F, I57M
Insertions 5insHHHHHSSGLVPRGSHMERPQLDSMSQDLSEALKEATKEVHIRAENSEFMRNFQKGQVSREGFKLVMASLYHIYTALEEEIERNKQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEAIPYTPATQHYVKRLHEVGGTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPSIDNPTKFKQLYRA
Deletions T236-E239
Sequence identity to UniProt 0.9318
auth_asym_id A
Structural gaps Exists
label_asym_ids of heme C
Available structure PDB
PDB ID:  pdb_00006j7i , Chain B 
Resolution 3.30 Å
Residue indices (Uniprot), coverage 10-853 (58-678), 0.99
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine:linker
Mutations (UniProt numbering) D3S, E6R, D7M, T8N, S9T, A10L, T11E, P13T, E14P, A15E, A17K, E18H, E19R, S21T, L22E, F23E, S24A, T25K, D27A, M28F, V29L, F31N, S32I, L33E, I34L, V35F, G36E, V37E, T39Q, Y40A, W41L, F42L, I43P, F44E, R45E, K46H, K48D, E49Q, E50S, I51A, P52S, E53Q, F54T, S55E, K56F, I57M
Insertions 5insHHHHHSSGLVPRGSHMERPQLDSMSQDLSEALKEATKEVHIRAENSEFMRNFQKGQVSREGFKLVMASLYHIYTALEEEIERNKQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEAIPYTPATQHYVKRLHEVGGTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPSIDNPTKFKQLYRA
Deletions T236-E239
Sequence identity to UniProt 0.9318
auth_asym_id B
Structural gaps Exists
label_asym_ids of heme F
Available structure PDB
PDB ID:  pdb_00006j79 , Chain A 
Resolution 3.33 Å
Residue indices (Uniprot), coverage 10-851 (58-678), 0.99
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine:linker
Mutations (UniProt numbering) D3S, D7A, S9K, A10E, T11V, M12H, P13I, E14R, V16E, A17N, E18S, V20F, S21M, L22R, F23N, S24F, T25Q, T26K, D27G, M28Q, L30S, F31R, S32E, L33G, I34F, V35K, G36L, L38M, T39A, Y40S, W41L, F42Y, I43H, F44I, R45Y, K46T, K47A, K48L, I51E, P52I, F54R, S55N, I57M
Insertions 5insHHHHHSSGLVPRGSHMERPQLDSMSQDLSEALK, 56insQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEAIPYTPATQHYVKRLHEVGGTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPSIDNPTKFKQLYRARMNTLEMTPEVKHRVTEEAKTAFLLNIELFEELQALLPEEHKDQSASQT
Deletions T236-E239
Sequence identity to UniProt 0.9362
auth_asym_id A
Structural gaps Exists
label_asym_ids of heme E
Available structure PDB
PDB ID:  pdb_00006j79 , Chain B 
Resolution 3.33 Å
Residue indices (Uniprot), coverage 10-851 (58-678), 0.99
Difference from Uniprot sequence deletion:engineered mutation:expression tag:initiating methionine:linker
Mutations (UniProt numbering) D3S, D7A, S9K, A10E, T11V, M12H, P13I, E14R, V16E, A17N, E18S, V20F, S21M, L22R, F23N, S24F, T25Q, T26K, D27G, M28Q, L30S, F31R, S32E, L33G, I34F, V35K, G36L, L38M, T39A, Y40S, W41L, F42Y, I43H, F44I, R45Y, K46T, K47A, K48L, I51E, P52I, F54R, S55N, I57M
Insertions 5insHHHHHSSGLVPRGSHMERPQLDSMSQDLSEALK, 56insQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEAIPYTPATQHYVKRLHEVGGTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPSIDNPTKFKQLYRARMNTLEMTPEVKHRVTEEAKTAFLLNIELFEELQALLPEEHKDQSASQT
Deletions T236-E239
Sequence identity to UniProt 0.9362
auth_asym_id B
Structural gaps Exists
label_asym_ids of heme H
Available structure PDB
PDB ID:  pdb_00003wkt , Chain A 
Resolution 4.30 Å
Residue indices (Uniprot), coverage 66-674 (58-678), 0.91
Difference from Uniprot sequence deletion:expression tag
Mutations (UniProt numbering) None
Insertions None
Deletions G2-I57, T236-E239
Sequence identity to UniProt 1.0000
auth_asym_id A
Missing residues 496ALA-502ARG
Structural gaps Exists
label_asym_ids of heme None
Available structure PDB and Modeled
RMSD of experimental residues after energy minimization 0.14 Å
PDB ID:  pdb_00003wkt , Chain B 
Resolution 4.30 Å
Residue indices (Uniprot), coverage 70-674 (58-678), 0.91
Difference from Uniprot sequence deletion:expression tag
Mutations (UniProt numbering) None
Insertions None
Deletions G2-I57, T236-E239
Sequence identity to UniProt 1.0000
auth_asym_id B
Missing residues 112ALA-122LEU:496ALA-501GLY
Structural gaps Exists
label_asym_ids of heme None
Available structure PDB and Modeled
RMSD of experimental residues after energy minimization 0.16 Å